The purpose of the trial is to observe the clinical efficacy of GS-441524 on naturally infected feline infectious peritonitis (FIP) cases, and to provide reference for clinical medication.
Twenty-five cats diagnosed with FIP at the Animal Hospital of China Agricultural University were recruited and randomly divided into a high-dose group (5 mg/(kg·d)) (12 cats) and a low-dose group (2.5 mg/(kg·d)). )) (13 animals), received daily subcutaneous injection of GS-441524 and recorded body weight, body temperature, pain response to the injected drug, and weekly laboratory and imaging examination results to evaluate the efficacy of the drug.
The treatment period was set as 4 weeks, and some cases extended the treatment time according to the actual situation. The results showed that a total of 20 cats with wet FIP and 3 cats with dry FIP completed the trial, and 2 cats withdrew. Treatment time is 4 to 18 weeks.
Among the 20 cats with wet FIP, 2 died within 1 week, 1 had increased body cavity effusion, and 17 had less effusion after 1 week of treatment, and disappeared after 2 to 3 weeks of treatment. Among the 3 cats with dry FIP, the mesenteric lymph nodes of 2 cats decreased after 1-2 weeks of treatment, and 1 had no significant change. The spirit and appetite of all the cats were improved within 3-5 days of medication; the body weight began to increase within 1-2 weeks; 5 cats with fever returned to normal after 2-3 days of medication; 14 cats with anemia were treated with 1 It returned to normal after ~10 weeks; 16 cats had increased white blood cell counts and decreased lymphocyte counts or increased neutrophil counts, which began to improve within 1–2 weeks of medication. The ratio of albumin to globulin (white globulin ratio) in the serum of all cats was <0.8, of which 9 had increased total protein, 4 had decreased albumin, and 14 had increased globulin, which began to improve after 1-2 weeks of treatment.
The 4-week drug effective rate and cure rate of the cats in the high-dose group were 81.8% and 60.0%, respectively, and the 4-week drug effective rate and 22.2% of the cats in the low-dose group were 75.0% and 22.2%, respectively. Before and after 4 weeks of treatment, except for the total number of white blood cells (WBC) and albumin (ALB), the cats in the high-dose group had significant or extremely significant differences (P<0.05; P<0.01); while the cats in the low-dose group Only weight differences were significant. In addition to hemoglobin (HGB), WBC and ALB, the drug had effects on body weight, red blood cells (RBC), hematocrit (HCT), lymphocytes (LYM), neutrophils (NEU), total protein (TP), The improvement of globulin (GLOB) and white ball ratio (A/G) was significantly different (P<0.05), and the effect of high-dose drug was better.
In conclusion, GS-441524 can effectively treat naturally infected FIP, and the high dose (5 mg/(kg·d)) has better efficacy, and the shortest treatment period is 4 weeks.